This book constitutes the proceedings of the 21st International Conference on Advanced Concepts for Intelligent Vision Systems, ACIVS 2023, held in Kumamoto, Japan, during August 2023. The 31 papers presented in this volume were carefully reviewed and selected from a total of 48 submissions. They were organized in topical sections named: Computer Vision, Affective Computing and Human Interactions, Managing the Biodiversity, Robotics and Drones, Machine Learning.