This book constitutes the refereed proceedings of the 26th Conference on Medical Image Understanding and Analysis, MIUA 2022, held in Cambridge, UK, in July 2022. The 65 full papers presented were carefully reviewed and selected from 95 submissions. They were organized according to following topical sections: biomarker detection; image registration, and reconstruction; image segmentation; generative models, biomedical simulation and modelling; classification; image enhancement, quality assessment, and data privacy; radiomics, predictive models, and quantitative imaging.